| Long-tailed Duck | Clangula | hyemalis | | 1 | Birds | Anatidae | Anseriformes | Aves | Animalia |
| Black Skimmer | Rynchops | niger | | 1 | Birds | Laridae | Charadriiformes | Aves | Animalia |
| Hudsonian Godwit | Limosa | haemastica | | 1 | Birds | Scolopacidae | Charadriiformes | Aves | Animalia |
| White-throated Sparrow | Zonotrichia | albicollis | | 1 | Birds | Emberizidae | Passeriformes | Aves | Animalia |
| Philadelphia Vireo | Vireo | philadelphicus | | 1 | Birds | Vireonidae | Passeriformes | Aves | Animalia |
| Twelve-spotted Tiger Beetle | Cicindela | duodecimguttata | | 1 | Tiger Beetles | Carabidae | Coleoptera | Insecta | Animalia |
| Pepper and Salt Skipper | Amblyscirtes | hegon | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Sachem | Atalopedes | huron | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Common Checkered Skipper | Burnsius | communis | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Hoary Edge | Cecropterus | lyciades | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Ocola Skipper | Panoquina | ocola | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Peck's Skipper | Polites | peckius | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Summer Azure | Celastrina | neglecta | | 2 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Eastern Tailed-Blue | Cupido | comyntas orientalis | | 1 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Bronze Copper | Lycaena | hyllus | | 1 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Silver-bordered Fritillary | Boloria | selene | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Monarch | Danaus | plexippus | | 2 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Common Buckeye | Junonia | coenia | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Appalachian Brown | Lethe | appalachia | | 2 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Little Wood Satyr | Megisto | cymela | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Pearl Crescent | Phyciodes | tharos | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Eastern Comma | Polygonia | comma | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Orange Sulphur | Colias | eurytheme | | 1 | Butterflies | Pieridae | Lepidoptera | Insecta | Animalia |
| Cloudless Sulphur | Phoebis | sennae | | 1 | Butterflies | Pieridae | Lepidoptera | Insecta | Animalia |
| Shadow Darner | Aeshna | umbrosa | | 1 | Dragonflies | Aeshnidae | Odonata | Insecta | Animalia |
| Common Green Darner | Anax | junius | | 1 | Dragonflies | Aeshnidae | Odonata | Insecta | Animalia |
| Spatterdock Darner | Rhionaeschna | mutata | | 1 | Dragonflies | Aeshnidae | Odonata | Insecta | Animalia |
| Superb Jewelwing | Calopteryx | amata | | 1 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| Appalachian Jewelwing | Calopteryx | angustipennis | | 1 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| Sparkling Jewelwing | Calopteryx | dimidiata | | 1 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| Ebony Jewelwing | Calopteryx | maculata | | 1 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| American Rubyspot | Hetaerina | americana | | 2 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| Eastern Red Damsel | Amphiagrion | saucium | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Blue-fronted Dancer | Argia | apicalis | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Seepage Dancer | Argia | bipunctulata | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Variable Dancer | Argia | fumipennis | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Powdered Dancer | Argia | moesta | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Blue-ringed Dancer | Argia | sedula | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Blue-tipped Dancer | Argia | tibialis | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Dusky Dancer | Argia | translata | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Aurora Damsel | Chromagrion | conditum | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Skimming Bluet | Enallagma | geminatum | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Hagen's Bluet | Enallagma | hageni | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Citrine Forktail | Ischnura | hastata | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Lilypad Forktail | Ischnura | kellicotti | | 2 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Fragile Forktail | Ischnura | posita | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Rambur's Forktail | Ischnura | ramburii | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Eastern Forktail | Ischnura | verticalis | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Sphagnum Sprite | Nehalennia | gracilis | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Southern Sprite | Nehalennia | integricollis | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Sedge Sprite | Nehalennia | irene | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Duckweed Firetail | Telebasis | byersi | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Brown Spiketail | Zoraena | bilineata | | 3 | Dragonflies | Cordulegastridae | Odonata | Insecta | Animalia |
| Delta-spotted Spiketail | Zoraena | diastatops | | 1 | Dragonflies | Cordulegastridae | Odonata | Insecta | Animalia |
| Twin-spotted Spiketail | Zoraena | maculata | | 1 | Dragonflies | Cordulegastridae | Odonata | Insecta | Animalia |
| Arrowhead Spiketail | Zoraena | obliqua | | 1 | Dragonflies | Cordulegastridae | Odonata | Insecta | Animalia |
| Beaverpond Baskettail | Epitheca | canis | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Common Baskettail | Epitheca | cynosura | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Robust Baskettail | Epitheca | spinosa | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Selys' Sundragon | Helocordulia | selysii | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Uhler's Sundragon | Helocordulia | uhleri | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Umber Shadowdragon | Neurocordulia | obsoleta | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Stygian Shadowdragon | Neurocordulia | yamaskanensis | | 1 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Clamp-tipped Emerald | Somatochlora | tenebrosa | | 2 | Dragonflies | Corduliidae | Odonata | Insecta | Animalia |
| Harpoon Clubtail | Phanogomphus | descriptus | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Lancet Clubtail | Phanogomphus | exilis | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Rapids Clubtail | Phanogomphus | quadricolor | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Dusky Clubtail | Phanogomphus | spicatus | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Great Spreadwing | Archilestes | grandis | | 4 | Damselflies | Lestidae | Odonata | Insecta | Animalia |
| Spotted Spreadwing | Lestes | congener | | 1 | Damselflies | Lestidae | Odonata | Insecta | Animalia |
| Elegant Spreadwing | Lestes | inaequalis | | 1 | Damselflies | Lestidae | Odonata | Insecta | Animalia |
| Slender Spreadwing | Lestes | rectangularis | | 2 | Damselflies | Lestidae | Odonata | Insecta | Animalia |
| Swamp Spreadwing | Lestes | vigilax | | 1 | Damselflies | Lestidae | Odonata | Insecta | Animalia |
| Chalk-fronted Corporal | Ladona | julia | | 1 | Dragonflies | Libellulidae | Odonata | Insecta | Animalia |
| Dot-tailed Whiteface | Leucorrhinia | intacta | | 1 | Dragonflies | Libellulidae | Odonata | Insecta | Animalia |
| Blue Dasher | Pachydiplax | longipennis | | 1 | Dragonflies | Libellulidae | Odonata | Insecta | Animalia |
| Gray Petaltail | Tachopteryx | thoreyi | | 1 | Dragonflies | Petaluridae | Odonata | Insecta | Animalia |
| Red Fox | Vulpes | vulpes | | 1 | Mammals | Canidae | Carnivora | Mammalia | Animalia |
| Dwarf Ginseng | Panax | trifolius | | 1 | Ginseng Family | Araliaceae | Apiales | Magnoliopsida | Plantae |
| Common Jack in the Pulpit | Arisaema | triphyllum | | 1 | Arum Family | Araceae | Arales | Magnoliopsida | Plantae |
| Skunk Cabbage | Symplocarpus | foetidus | | 1 | Arum Family | Araceae | Arales | Magnoliopsida | Plantae |
| Early Yellowrocket | Barbarea | verna | | 1 | Mustard Family | Brassicaceae | Brassicales | Magnoliopsida | Plantae |
| Garden Yellowrocket | Barbarea | vulgaris | | 1 | Mustard Family | Brassicaceae | Brassicales | Magnoliopsida | Plantae |
| Pennsylvania Bittercress | Cardamine | pensylvanica | | 1 | Mustard Family | Brassicaceae | Brassicales | Magnoliopsida | Plantae |
| Star Chickweed | Stellaria | pubera | | 1 | Pink Family | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Virginia Springbeauty | Claytonia | virginica | | 1 | Montia Family | Montiaceae | Caryophyllales | Magnoliopsida | Plantae |
| Flowering Dogwood | Cornus | florida | | 1 | Dogwood Family | Cornaceae | Cornales | Magnoliopsida | Plantae |
| Mountain Laurel | Kalmia | latifolia | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| American Hornbeam | Carpinus | caroliniana | | 1 | Birch Family | Betulaceae | Fagales | Magnoliopsida | Plantae |
| Yellow Trout Lily | Erythronium | americanum ssp. americanum | | 1 | Lily Family | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Indian Strawberry | Potentilla | indica | | 1 | Rose Family | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Jetbead | Rhodotypos | scandens | | 1 | Rose Family | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Azure Bluets | Houstonia | caerulea | | 1 | Madder Family | Rubiaceae | Rubiales | Magnoliopsida | Plantae |
| Sensitive Fern | Onoclea | sensibilis | | 1 | Ferns | Onocleaceae | Polypodiales | Polypodiopsida | Plantae |
| Common Polypody | Polypodium | virginianum | | 1 | Ferns | Polypodiaceae | Polypodiales | Polypodiopsida | Plantae |