| American Bullfrog | Lithobates | catesbeianus | | 3 | Amphibians | Ranidae | Anura | Amphibia | Animalia |
| Green Frog | Lithobates | clamitans | | 2 | Amphibians | Ranidae | Anura | Amphibia | Animalia |
| Golden Eagle | Aquila | chrysaetos | | 1 | Birds | Accipitridae | Accipitriformes | Aves | Animalia |
| Bald Eagle | Haliaeetus | leucocephalus | | 1 | Birds | Accipitridae | Accipitriformes | Aves | Animalia |
| Osprey | Pandion | haliaetus | | 2 | Birds | Pandioidae | Accipitriformes | Aves | Animalia |
| Ross's Goose | Anser | rossii | | 1 | Birds | Anatidae | Anseriformes | Aves | Animalia |
| Bufflehead | Bucephala | albeola | | 1 | Birds | Anatidae | Anseriformes | Aves | Animalia |
| Hooded Merganser | Lophodytes | cucullatus | | 1 | Birds | Anatidae | Anseriformes | Aves | Animalia |
| Laughing Gull | Leucophaeus | atricilla | | 1 | Birds | Laridae | Charadriiformes | Aves | Animalia |
| American Kestrel | Falco | sparverius | | 1 | Birds | Falconidae | Falconiformes | Aves | Animalia |
| Common Loon | Gavia | immer | | 1 | Birds | Gaviidae | Gaviiformes | Aves | Animalia |
| Northern Cardinal | Cardinalis | cardinalis | | 1 | Birds | Cardinalidae | Passeriformes | Aves | Animalia |
| Evening Grosbeak | Coccothraustes | vespertinus | | 1 | Birds | Fringillidae | Passeriformes | Aves | Animalia |
| Kentucky Warbler | Geothlypis | formosa | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Common Yellowthroat | Geothlypis | trichas | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Black-and-white Warbler | Mniotilta | varia | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Myrtle Warbler | Setophaga | coronata coronata | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Chestnut-sided Warbler | Setophaga | pensylvanica | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Pine Warbler | Setophaga | pinus | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Ruby-crowned Kinglet | Corthylio | calendula | | 1 | Birds | Regulidae | Passeriformes | Aves | Animalia |
| Winter Wren | Troglodytes | hiemalis | | 1 | Birds | Troglodytidae | Passeriformes | Aves | Animalia |
| Eastern Kingbird | Tyrannus | tyrannus | | 1 | Birds | Tyrannidae | Passeriformes | Aves | Animalia |
| Yellow-throated Vireo | Vireo | flavifrons | | 1 | Birds | Vireonidae | Passeriformes | Aves | Animalia |
| Great Blue Heron | Ardea | herodias | | 1 | Birds | Ardeidae | Pelecaniformes | Aves | Animalia |
| Green Heron | Butorides | virescens | | 1 | Birds | Ardeidae | Pelecaniformes | Aves | Animalia |
| American White Pelican | Pelecanus | erythrorhynchos | | 1 | Birds | Pelecanidae | Pelecaniformes | Aves | Animalia |
| Hairy Woodpecker | Dryobates | villosus | | 1 | Birds | Picidae | Piciformes | Aves | Animalia |
| Pileated Woodpecker | Dryocopus | pileatus | | 1 | Birds | Picidae | Piciformes | Aves | Animalia |
| Barred Owl | Strix | varia | | 1 | Birds | Strigidae | Strigiformes | Aves | Animalia |
| Locust Borer | Megacyllene | robiniae | | 1 | Long-horned Beetles | Cerambycidae | Coleoptera | Insecta | Animalia |
| No Common Name | Acilius | mediatus | | 1 | Predaceous Diving Beetles | Dytiscidae | Coleoptera | Insecta | Animalia |
| Patent Leather Beetle | Odontotaenius | disjunctus | | 1 | Scarab Beetles | Passalidae | Coleoptera | Insecta | Animalia |
| Red-footed Cannibalfly | Promachus | rufipes | | 3 | Robber Flies | Asilidae | Diptera | Insecta | Animalia |
| Maize Calligrapher | Toxomerus | politus | | 1 | Syrphid Flies | Syrphidae | Diptera | Insecta | Animalia |
| Green Stink Bug | Chinavia | hilaris | | 1 | Stink Bugs | Pentatomidae | Hemiptera | Insecta | Animalia |
| Honey Bee | Apis | mellifera | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Two-spotted Bumble Bee | Bombus | bimaculatus | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Northern Carpenter Bee | Xylocopa | virginica | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Round Bullet Gall Wasp | Disholcaspis | quercusglobulus | | 1 | Gall Wasps | Cynipidae | Hymenoptera | Insecta | Animalia |
| Rusty Spider Wasp (ferrugineus) | Tachypompilus | ferrugineus ferrugineus | | 1 | Spider Wasps | Pompilidae | Hymenoptera | Insecta | Animalia |
| No Common Name | Parancistrocerus | fulvipes | | 1 | Vespid Wasps | Vespidae | Hymenoptera | Insecta | Animalia |
| Discolored Renia Moth | Renia | discoloralis | | 1 | Moths | Erebidae | Lepidoptera | Insecta | Animalia |
| Lunate Zale Moth | Zale | lunata | | 1 | Moths | Erebidae | Lepidoptera | Insecta | Animalia |
| Grayish Zanclognatha Moth | Zanclognatha | pedipilalis | | 1 | Moths | Erebidae | Lepidoptera | Insecta | Animalia |
| Tulip-tree Beauty Moth | Epimecis | hortaria | | 1 | Moths | Geometridae | Lepidoptera | Insecta | Animalia |
| Least Skipper | Ancyloxypha | numitor | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Sachem | Atalopedes | huron | | 9 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Common Checkered Skipper | Burnsius | communis | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Horace's Duskywing | Erynnis | horatius | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Fiery Skipper | Hylephila | phyleus | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Zabulon Skipper | Lon | zabulon | | 2 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Ocola Skipper | Panoquina | ocola | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Little Glassywing | Vernia | verna | | 1 | Butterflies | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Red-banded Hairstreak | Calycopis | cecrops | | 1 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Eastern Tailed-Blue | Cupido | comyntas orientalis | | 1 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Gray Hairstreak | Strymon | melinus | | 1 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Drexel's Datana Moth | Datana | drexelii | | 1 | Moths | Notodontidae | Lepidoptera | Insecta | Animalia |
| Monarch | Danaus | plexippus | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Northern Pearly-eye | Lethe | anthedon | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Appalachian Brown | Lethe | appalachia | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Red-spotted Purple | Limenitis | arthemis astyanax | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Pearl Crescent | Phyciodes | tharos | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Red Admiral | Vanessa | atalanta | | 2 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Eastern Tiger Swallowtail | Pterourus | glaucus | | 1 | Butterflies | Papilionidae | Lepidoptera | Insecta | Animalia |
| Spicebush Swallowtail | Pterourus | troilus | | 1 | Butterflies | Papilionidae | Lepidoptera | Insecta | Animalia |
| Sleepy Orange | Abaeis | nicippe | | 2 | Butterflies | Pieridae | Lepidoptera | Insecta | Animalia |
| Cabbage White | Pieris | rapae | | 1 | Butterflies | Pieridae | Lepidoptera | Insecta | Animalia |
| Tulip-tree Silkmoth | Callosamia | angulifera | | 1 | Moths | Saturniidae | Lepidoptera | Insecta | Animalia |
| Regal Moth | Citheronia | regalis | | 1 | Moths | Saturniidae | Lepidoptera | Insecta | Animalia |
| Pandorus Sphinx | Eumorpha | pandorus | | 1 | Moths | Sphingidae | Lepidoptera | Insecta | Animalia |
| Aurora Damsel | Chromagrion | conditum | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Vesper Bluet | Enallagma | vesperum | | 1 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Lilypad Forktail | Ischnura | kellicotti | | 2 | Damselflies | Coenagrionidae | Odonata | Insecta | Animalia |
| Calico Pennant | Celithemis | elisa | | 1 | Dragonflies | Libellulidae | Odonata | Insecta | Animalia |
| Halloween Pennant | Celithemis | eponina | | 1 | Dragonflies | Libellulidae | Odonata | Insecta | Animalia |
| Common Pondhawk | Erythemis | simplicicollis | | 1 | Dragonflies | Libellulidae | Odonata | Insecta | Animalia |
| Stream Cruiser | Didymops | transversa | | 1 | Dragonflies | Macromiidae | Odonata | Insecta | Animalia |
| Wrinkled Grasshopper | Hippiscus | ocelote | | 1 | Grasshoppers | Acrididae | Orthoptera | Insecta | Animalia |
| Common Raccoon | Procyon | lotor | | 1 | Mammals | Procyonidae | Carnivora | Mammalia | Animalia |
| Delmarva Fox Squirrel | Sciurus | niger cinereus | | 2 | Mammals | Sciuridae | Rodentia | Mammalia | Animalia |
| Eastern Hog-nosed Snake | Heterodon | platirhinos | | 1 | Reptiles | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Ribbonsnake | Thamnophis | saurita | | 1 | Reptiles | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Copperhead | Agkistrodon | contortrix | | 1 | Reptiles | Crotalidae | Squamata | Reptilia | Animalia |
| Eastern Fence Lizard | Sceloporus | undulatus | | 1 | Reptiles | Phrynosomatidae | Squamata | Reptilia | Animalia |
| Green Gill Mushroom | Chlorophyllum | molybdites | | 1 | Fungi | Agaricaceae | Agaricales | Agaricomycetes | Fungi |
| Cedar Apple Rust | Gymnosporangium | juniperi-virginianae | | 1 | Fungi | Pucciniaceae | Pucciniales | Pucciniomycetes | Fungi |
| Black Elderberry | Sambucus | canadensis | | 1 | Moschatel Family | Adoxaceae | Dipsacales | Magnoliopsida | Plantae |
| Trailing Arbutus | Epigaea | repens | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Indian Pipe | Monotropa | uniflora | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Flowering Spurge | Euphorbia | corollata | | 1 | Spurge Family | Euphorbiaceae | Euphorbiales | Magnoliopsida | Plantae |
| Groundnut | Apios | americana | | 1 | Pea Family | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Perplexed Tick-trefoil | Desmodium | perplexum | | 1 | Pea Family | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Spatterdock | Nuphar | advena | | 1 | Water-lily Family | Nymphaeaceae | Nymphaeales | Magnoliopsida | Plantae |
| Woolgrass | Scirpus | cyperinus | | 1 | Sedge Family | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Cinnamon Fern | Osmundastrum | cinnamomeum | | 1 | Ferns | Osmundaceae | Osmundales | Polypodiopsida | Plantae |