| Long-tailed Salamander | Eurycea | longicauda longicauda | | 1 | Amphibians | Plethodontidae | Caudata | Amphibia | Animalia |
| Eastern Red-backed Salamander | Plethodon | cinereus | | 1 | Amphibians | Plethodontidae | Caudata | Amphibia | Animalia |
| No Common Name | Barronopsis | jeffersi | | 1 | Spiders | Agelenidae | Araneae | Arachnida | Animalia |
| Goldenrod Soldier Beetle | Chauliognathus | pensylvanicus | | 1 | Soldier Beetles | Cantharidae | Coleoptera | Insecta | Animalia |
| Locust Borer | Megacyllene | robiniae | | 1 | Long-horned Beetles | Cerambycidae | Coleoptera | Insecta | Animalia |
| Red Milkweed Beetle | Tetraopes | tetrophthalmus | | 1 | Long-horned Beetles | Cerambycidae | Coleoptera | Insecta | Animalia |
| July Comets | Photuris | lucicrescens | | 1 | Fireflies | Lampyridae | Coleoptera | Insecta | Animalia |
| No Common Name | Photuris | sp. | | 1 | Fireflies | Lampyridae | Coleoptera | Insecta | Animalia |
| No Common Name | Lytta | aenea | | 1 | Blister Beetles | Meloidae | Coleoptera | Insecta | Animalia |
| No Common Name | Meloe | campanicollis | | 1 | Blister Beetles | Meloidae | Coleoptera | Insecta | Animalia |
| Green Stink Bug | Chinavia | hilaris | | 1 | Stink Bugs | Pentatomidae | Hemiptera | Insecta | Animalia |
| Hairy-footed Flower Bee | Anthophora | villosula | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Black-and-gold Bumble Bee | Bombus | auricomus | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Golden Northern Bumble Bee | Bombus | fervidus | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Brown-belted Bumble Bee | Bombus | griseocollis | | 1 | Bees | Apidae | Hymenoptera | Insecta | Animalia |
| Blue-winged Wasp | Scolia | dubia | | 1 | Scoliid Wasps | Scoliidae | Hymenoptera | Insecta | Animalia |
| Spotted Beet Webworm Moth | Hymenia | perspectalis | | 1 | Moths | Crambidae | Lepidoptera | Insecta | Animalia |
| Great Spangled Fritillary | Argynnis | cybele | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Pearl Crescent | Phyciodes | tharos | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Question Mark | Polygonia | interrogationis | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Zebra Swallowtail | Eurytides | marcellus | | 1 | Butterflies | Papilionidae | Lepidoptera | Insecta | Animalia |
| Luna Moth | Actias | luna | | 1 | Moths | Saturniidae | Lepidoptera | Insecta | Animalia |
| Rough Greensnake | Opheodrys | aestivus | | 1 | Reptiles | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 1 | Reptiles | Emydidae | Testudines | Reptilia | Animalia |
| Coral-pink Merulius | Byssomerulius | incarnatus | | 1 | Fungi | Irpicaceae | Polyporales | Agaricomycetes | Fungi |
| Rattlesnake Weed | Hieracium | venosum | | 1 | Aster Family | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Downy Lobelia | Lobelia | puberula | | 1 | Bellflower Family | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| Spatula-leaved Sundew | Drosera | intermedia | | 1 | Carnivorous Plants | Droseraceae | Caryophyllales | Magnoliopsida | Plantae |
| Wart-removing Herb | Murdannia | keisak | | 1 | Spiderwort Family | Commelinaceae | Commelinales | Magnoliopsida | Plantae |
| Trailing Arbutus | Epigaea | repens | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Green Milkweed | Asclepias | viridiflora | | 1 | Milkweed Family | Apocynaceae | Gentianales | Magnoliopsida | Plantae |
| Carolina Wild Petunia | Ruellia | caroliniensis | | 1 | Acanthus Family | Acanthaceae | Lamiales | Magnoliopsida | Plantae |
| Common Selfheal | Prunella | vulgaris | | 1 | Mint Family | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Downy Rattlesnake Plantain | Goodyera | pubescens | | 2 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Eastern Gamagrass | Tripsacum | dactyloides | | 1 | Grasses | Poaceae | Poales | Magnoliopsida | Plantae |
| Partridgeberry | Mitchella | repens | | 1 | Madder Family | Rubiaceae | Rubiales | Magnoliopsida | Plantae |
| American Mistletoe | Phoradendron | leucarpum | | 1 | Sandalwood Family | Santalaceae | Santalales | Magnoliopsida | Plantae |
| Wild Blue Phlox | Phlox | divaricata var. divaricata | | 1 | Phlox Family | Polemoniaceae | Solanales | Magnoliopsida | Plantae |
| Creeping Cucumber | Melothria | pendula | | 1 | Cucumber Family | Cucurbitaceae | Violales | Magnoliopsida | Plantae |
| Netted Chainfern | Lorinseria | areolata | | 1 | Ferns | Blechnaceae | Polypodiales | Polypodiopsida | Plantae |
| Sensitive Fern | Onoclea | sensibilis | | 1 | Ferns | Onocleaceae | Polypodiales | Polypodiopsida | Plantae |