| American Eel | Anguilla | rostrata | | 2 | Fish | Anguillidae | Anguilliformes | Actinopterygii | Animalia |
| White Sucker | Catostomus | commersonii | | 2 | Fish | Catostomidae | Cypriniformes | Actinopterygii | Animalia |
| Creek Chubsucker | Erimyzon | oblongus | | 2 | Fish | Catostomidae | Cypriniformes | Actinopterygii | Animalia |
| Northern Hogsucker | Hypentelium | nigricans | | 3 | Fish | Catostomidae | Cypriniformes | Actinopterygii | Animalia |
| Central Stoneroller | Campostoma | anomalum | | 6 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Rosyside Dace | Clinostomus | funduloides | | 2 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Silverjaw Minnow | Ericymba | buccatus | | 1 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Cutlips Minnow | Exoglossum | maxillingua | | 3 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Common Shiner | Luxilus | cornutus | | 3 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Pearl Dace | Margariscus | margarita | | 1 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Swallowtail Shiner | Miniellus | procne | | 1 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Bluntnose Minnow | Pimephales | notatus | | 8 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Blacknose Dace | Rhinichthys | atratulus | | 5 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Longnose Dace | Rhinichthys | cataractae | | 6 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Creek Chub | Semotilus | atromaculatus | | 3 | Fish | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Sheepshead Minnow | Cyprinodon | variegatus | | 1 | Fish | Cyprinodontidae | Cyprinodontiformes | Actinopterygii | Animalia |
| Banded Killifish | Fundulus | diaphanus | | 3 | Fish | Fundulidae | Cyprinodontiformes | Actinopterygii | Animalia |
| Mummichog | Fundulus | heteroclitus | | 1 | Fish | Fundulidae | Cyprinodontiformes | Actinopterygii | Animalia |
| Striped Killifish | Fundulus | majalis | | 1 | Fish | Fundulidae | Cyprinodontiformes | Actinopterygii | Animalia |
| Eastern Mudminnow | Umbra | pygmaea | | 3 | Fish | Umbridae | Esociformes | Actinopterygii | Animalia |
| Rock Bass | Ambloplites | rupestris | | 3 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Bluespotted Sunfish | Enneacanthus | gloriosus | | 1 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Redbreast Sunfish | Lepomis | auritus | | 9 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Green Sunfish | Lepomis | cyanellus | | 5 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Pumpkinseed | Lepomis | gibbosus | | 1 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Warmouth | Lepomis | gulosus | | 2 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Bluegill | Lepomis | macrochirus | | 5 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Longear Sunfish | Lepomis | megalotis | | 4 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Smallmouth Bass | Micropterus | dolomieu | | 4 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Largemouth Bass | Micropterus | nigricans | | 2 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Black Crappie | Pomoxis | nigromaculatus | | 1 | Fish | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Skilletfish | Gobiesox | strumosus | | 1 | Fish | Gobiesocidae | Perciformes | Actinopterygii | Animalia |
| Greenside Darter | Etheostoma | blennioides | | 10 | Fish | Percidae | Perciformes | Actinopterygii | Animalia |
| Rainbow Darter | Etheostoma | caeruleum | | 16 | Fish | Percidae | Perciformes | Actinopterygii | Animalia |
| Fantail Darter | Etheostoma | flabellare | | 15 | Fish | Percidae | Perciformes | Actinopterygii | Animalia |
| Tessellated Darter | Etheostoma | olmstedi | | 1 | Fish | Percidae | Perciformes | Actinopterygii | Animalia |
| Shield Darter | Percina | peltata | | 1 | Fish | Percidae | Perciformes | Actinopterygii | Animalia |
| Pirate Perch | Aphredoderus | sayanus | | 1 | Fish | Aphredoderidae | Percopsiformes | Actinopterygii | Animalia |
| Rainbow Trout | Oncorhynchus | mykiss | | 1 | Fish | Salmonidae | Salmoniformes | Actinopterygii | Animalia |
| Brown Trout | Salmo | trutta | | 2 | Fish | Salmonidae | Salmoniformes | Actinopterygii | Animalia |
| Blue Ridge Sculpin | Cottus | caeruleomentum | | 1 | Fish | Cottidae | Scorpaeniformes | Actinopterygii | Animalia |
| Potomac Sculpin | Cottus | girardi | | 2 | Fish | Cottidae | Scorpaeniformes | Actinopterygii | Animalia |
| Yellow Bullhead | Ameiurus | natalis | | 2 | Fish | Ictaluridae | Siluriformes | Actinopterygii | Animalia |
| Margined Madtom | Noturus | insignis | | 2 | Fish | Ictaluridae | Siluriformes | Actinopterygii | Animalia |
| American Toad | Anaxyrus | americanus americanus | | 2 | Amphibians | Bufonidae | Anura | Amphibia | Animalia |
| Fowler's Toad | Anaxyrus | fowleri | | 2 | Amphibians | Bufonidae | Anura | Amphibia | Animalia |
| Spring Peeper | Pseudacris | crucifer crucifer | | 1 | Amphibians | Hylidae | Anura | Amphibia | Animalia |
| Green Frog | Lithobates | clamitans | | 2 | Amphibians | Ranidae | Anura | Amphibia | Animalia |
| Pickerel Frog | Lithobates | palustris | | 1 | Amphibians | Ranidae | Anura | Amphibia | Animalia |
| Southern Leopard Frog | Lithobates | sphenocephalus utricularius | | 1 | Amphibians | Ranidae | Anura | Amphibia | Animalia |
| Northern Two-lined Salamander | Eurycea | bislineata | | 1 | Amphibians | Plethodontidae | Caudata | Amphibia | Animalia |
| Long-tailed Salamander | Eurycea | longicauda longicauda | | 1 | Amphibians | Plethodontidae | Caudata | Amphibia | Animalia |
| Red Salamander | Pseudotriton | ruber | | 3 | Amphibians | Plethodontidae | Caudata | Amphibia | Animalia |
| No Common Name | Leiobunum | vittatum | | 1 | Harvestmen | Sclerosomatidae | Opiliones | Arachnida | Animalia |
| Bald Eagle | Haliaeetus | leucocephalus | | 1 | Birds | Accipitridae | Accipitriformes | Aves | Animalia |
| Eastern Bluebird | Sialia | sialis | | 1 | Birds | Turdidae | Passeriformes | Aves | Animalia |
| Green Heron | Butorides | virescens | | 1 | Birds | Ardeidae | Pelecaniformes | Aves | Animalia |
| Least Brook Lamprey | Lampetra | aepyptera | | 1 | Fish | Petromyzontidae | Petromyzontiformes | Cephalaspidomorphi | Animalia |
| Pointed Campeloma | Campeloma | decisum | | 1 | Freshwater Snails | Viviparidae | Architaenioglossa | Gastropoda | Animalia |
| Crested Mudalia | Leptoxis | carinata | | 1 | Freshwater Snails | Pleuroceridae | Neotaenioglossa | Gastropoda | Animalia |
| No Common Name | Dineutus | discolor | | 1 | Whirligig Beetles | Gyrinidae | Coleoptera | Insecta | Animalia |
| No Common Name | Limnohydrobius | melaenus | | 1 | Water Scavenger Beetles | Hydrophilidae | Coleoptera | Insecta | Animalia |
| No Common Name | Psephenus | herricki | | 1 | Water Penny Beetles | Psephenidae | Coleoptera | Insecta | Animalia |
| Spotted Lanternfly | Lycorma | delicatula | | 1 | Planthoppers | Fulgoridae | Hemiptera | Insecta | Animalia |
| No Common Name | Ranatra | sp. | | 1 | Waterscorpions | Nepidae | Hemiptera | Insecta | Animalia |
| Wheel Bug | Arilus | cristatus | | 1 | Assassin Bugs | Reduviidae | Hemiptera | Insecta | Animalia |
| Eastern Boxelder Bug | Boisea | trivittata | | 1 | Scentless Plant Bugs | Rhopalidae | Hemiptera | Insecta | Animalia |
| European Hornet | Vespa | crabro | | 1 | Vespid Wasps | Vespidae | Hymenoptera | Insecta | Animalia |
| Orange-banded Tripudia Moth | Tripudia | flavofasciata | | 1 | Moths | Noctuidae | Lepidoptera | Insecta | Animalia |
| Hackberry Emperor | Asterocampa | celtis | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Silvery Checkerspot | Chlosyne | nycteis | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Zebra Swallowtail | Eurytides | marcellus | | 1 | Butterflies | Papilionidae | Lepidoptera | Insecta | Animalia |
| Ebony Jewelwing | Calopteryx | maculata | | 1 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| Dragonhunter | Hagenius | brevistylus | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Lancet Clubtail | Phanogomphus | exilis | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| American Beaver | Castor | canadensis | | 1 | Mammals | Castoridae | Rodentia | Mammalia | Animalia |
| Ring-necked Snake | Diadophis | punctatus | | 1 | Reptiles | Colubridae | Squamata | Reptilia | Animalia |
| Northern Watersnake | Nerodia | sipedon sipedon | | 2 | Reptiles | Colubridae | Squamata | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 1 | Reptiles | Emydidae | Testudines | Reptilia | Animalia |
| Eastern Musk Turtle | Sternotherus | odoratus | | 2 | Reptiles | Kinosternidae | Testudines | Reptilia | Animalia |
| American Eelgrass | Vallisneria | americana | | 1 | Tape-grass Family | Hydrocharitaceae | Hydrocharitales | Magnoliopsida | Plantae |
| Pawpaw | Asimina | triloba | | 1 | Custard-apple Family | Annonaceae | Magnoliales | Magnoliopsida | Plantae |