| American Toad | Anaxyrus | americanus americanus | | 1 | Amphibians | Bufonidae | Anura | Amphibia | Animalia |
| Fowler's Toad | Anaxyrus | fowleri | | 1 | Amphibians | Bufonidae | Anura | Amphibia | Animalia |
| White-breasted Nuthatch | Sitta | carolinensis | | 1 | Birds | Sittidae | Passeriformes | Aves | Animalia |
| Double-crested Cormorant | Nannopterum | auritum | | 1 | Birds | Phalacrocoracidae | Suliformes | Aves | Animalia |
| Common Raccoon | Procyon | lotor | | 1 | Mammals | Procyonidae | Carnivora | Mammalia | Animalia |
| Common Five-lined Skink | Plestiodon | fasciatus | | 1 | Reptiles | Scincidae | Squamata | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 0 | Reptiles | Emydidae | Testudines | Reptilia | Animalia |
| Red Chanterelle | Cantharellus | cinnabarinus | | 0 | Fungi | Cantharellaceae | Cantharellales | Agaricomycetes | Fungi |
| Mountain Laurel Leaf Spot | Mycosphaerella | colorata | | 1 | Fungi | Mycosphaerellaceae | Capnodiales | Dothideomycetes | Fungi |
| Southern Ground Cedar | Diphasiastrum | digitatum | | 1 | Clubmoss Family | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Common Cow-parsnip | Heracleum | maximum | | 1 | Carrot Family | Apiaceae | Apiales | Magnoliopsida | Plantae |
| False Solomon's-seal | Maianthemum | racemosum | | 1 | Asparagus Family | Asparagaceae | Asparagales | Magnoliopsida | Plantae |
| Late-flowering Thoroughwort | Eupatorium | serotinum | | 1 | Aster Family | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Wild Pink | Silene | caroliniana ssp. pensylvanica | | 1 | Pink Family | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Virginia Springbeauty | Claytonia | virginica | | 1 | Montia Family | Montiaceae | Caryophyllales | Magnoliopsida | Plantae |
| Japanese Knotweed | Reynoutria | japonica | | 1 | Buckwheat Family | Polygonaceae | Caryophyllales | Magnoliopsida | Plantae |
| Oriental Bittersweet | Celastrus | orbiculatus | | 1 | Bittersweet Family | Celastraceae | Celastrales | Magnoliopsida | Plantae |
| Black Elderberry | Sambucus | canadensis | | 2 | Moschatel Family | Adoxaceae | Dipsacales | Magnoliopsida | Plantae |
| Spotted Wintergreen | Chimaphila | maculata | | 2 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Trailing Arbutus | Epigaea | repens | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Mountain Laurel | Kalmia | latifolia | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Early Lowbush Blueberry | Vaccinium | pallidum | | 1 | Heath Family | Ericaceae | Ericales | Magnoliopsida | Plantae |
| American Hornbeam | Carpinus | caroliniana | | 1 | Birch Family | Betulaceae | Fagales | Magnoliopsida | Plantae |
| Mockernut Hickory | Carya | tomentosa | | 1 | Walnut Family | Juglandaceae | Juglandales | Magnoliopsida | Plantae |
| Virginia Bluebells | Mertensia | virginica | | 2 | Borage Family | Boraginaceae | Lamiales | Magnoliopsida | Plantae |
| Spotted Beebalm | Monarda | punctata | | 1 | Mint Family | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Beechdrops | Epifagus | virginiana | | 1 | Broom-rape Family | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Wild Yam | Dioscorea | villosa | | 1 | Yam Family | Dioscoreaceae | Liliales | Magnoliopsida | Plantae |
| Yellow Trout Lily | Erythronium | americanum ssp. americanum | | 3 | Lily Family | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Indian Cucumber | Medeola | virginiana | | 1 | Lily Family | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Red Trillium | Trillium | erectum | | 1 | Bunchflower Family | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Sessile Trillium | Trillium | sessile | | 1 | Bunchflower Family | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Pawpaw | Asimina | triloba | | 1 | Custard-apple Family | Annonaceae | Magnoliales | Magnoliopsida | Plantae |
| Seedbox | Ludwigia | alternifolia | | 1 | Evening Primrose Family | Onagraceae | Myrtales | Magnoliopsida | Plantae |
| Curly Pondweed | Potamogeton | crispus | | 1 | Pondweed Family | Potamogetonaceae | Najadales | Magnoliopsida | Plantae |
| Spatterdock | Nuphar | advena | | 1 | Water-lily Family | Nymphaeaceae | Nymphaeales | Magnoliopsida | Plantae |
| American White Water-lily | Nymphaea | odorata ssp. odorata | | 1 | Water-lily Family | Nymphaeaceae | Nymphaeales | Magnoliopsida | Plantae |
| Puttyroot | Aplectrum | hyemale | | 1 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Pink Lady's Slipper | Cypripedium | acaule | | 0 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Downy Rattlesnake Plantain | Goodyera | pubescens | | 1 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Cranefly Orchid | Tipularia | discolor | | 1 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Squirrel Corn | Dicentra | canadensis | | 0 | Poppy Family | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Dutchman's Breeches | Dicentra | cucullaria | | 2 | Poppy Family | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Twinleaf | Jeffersonia | diphylla | | 0 | Barberry Family | Berberidaceae | Ranunculales | Magnoliopsida | Plantae |
| Mayapple | Podophyllum | peltatum | | 3 | Barberry Family | Berberidaceae | Ranunculales | Magnoliopsida | Plantae |
| Jetbead | Rhodotypos | scandens | | 1 | Rose Family | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Black Raspberry | Rubus | occidentalis | | 1 | Rose Family | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Azure Bluets | Houstonia | caerulea | | 1 | Madder Family | Rubiaceae | Rubiales | Magnoliopsida | Plantae |
| Partridgeberry | Mitchella | repens | | 1 | Madder Family | Rubiaceae | Rubiales | Magnoliopsida | Plantae |
| Striped White Violet | Viola | striata | | 1 | Violet Family | Violaceae | Violales | Magnoliopsida | Plantae |
| Sensitive Fern | Onoclea | sensibilis | | 1 | Ferns | Onocleaceae | Polypodiales | Polypodiopsida | Plantae |
| New York Fern | Parathelypteris | noveboracensis | | 1 | Ferns | Thelypteridaceae | Polypodiales | Polypodiopsida | Plantae |