| Kentucky Warbler | Geothlypis | formosa | | 1 | Birds | Parulidae | Passeriformes | Aves | Animalia |
| Six-spotted Tiger Beetle | Cicindela | sexguttata | | 1 | Tiger Beetles | Carabidae | Coleoptera | Insecta | Animalia |
| American Copper | Lycaena | phlaeas | | 1 | Butterflies | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Common Buckeye | Junonia | coenia | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Eastern Comma | Polygonia | comma | | 1 | Butterflies | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Zebra Swallowtail | Eurytides | marcellus | | 1 | Butterflies | Papilionidae | Lepidoptera | Insecta | Animalia |
| Ebony Jewelwing | Calopteryx | maculata | | 1 | Damselflies | Calopterygidae | Odonata | Insecta | Animalia |
| Black-shouldered Spinyleg | Dromogomphus | spinosus | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Dragonhunter | Hagenius | brevistylus | | 1 | Dragonflies | Gomphidae | Odonata | Insecta | Animalia |
| Ring-necked Snake | Diadophis | punctatus | | 1 | Reptiles | Colubridae | Squamata | Reptilia | Animalia |
| Snapping Turtle | Chelydra | serpentina | | 1 | Reptiles | Chelydridae | Testudines | Reptilia | Animalia |
| Eastern Box Turtle | Terrapene | carolina carolina | | 1 | Reptiles | Emydidae | Testudines | Reptilia | Animalia |
| Wild Sarsaparilla | Aralia | nudicaulis | | 1 | Ginseng Family | Araliaceae | Apiales | Magnoliopsida | Plantae |
| Dwarf Ginseng | Panax | trifolius | | 1 | Ginseng Family | Araliaceae | Apiales | Magnoliopsida | Plantae |
| False Solomon's-seal | Maianthemum | racemosum | | 1 | Asparagus Family | Asparagaceae | Asparagales | Magnoliopsida | Plantae |
| Rattlesnake Weed | Hieracium | venosum | | 1 | Aster Family | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Lyrate Rockcress | Arabidopsis | lyrata ssp. lyrata | | 1 | Mustard Family | Brassicaceae | Brassicales | Magnoliopsida | Plantae |
| Widowsfrill | Silene | stellata | | 1 | Pink Family | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Pink Lady's Slipper | Cypripedium | acaule | | 2 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Downy Rattlesnake Plantain | Goodyera | pubescens | | 1 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Southern Palegreen Orchid (flava) | Platanthera | flava var. flava | | 1 | Orchids | Orchidaceae | Orchidales | Magnoliopsida | Plantae |